IDENTIFICATION AND VERIFICATION OF DIFFERENTIAL DISPLAY CDNAS USING GENE-SPECIFIC PRIMERS AND HYBRIDIZATION ARRAYS

Identification and Verification of Differential Display cDNAs Using Gene-Specific Primers and Hybridization Arrays

An accurate and streamlined approach to differential display (DD) band identification and verification is described.To minimize false positives, the strategy avoids the use of impure Northern blot probes obtained from PCR-amplified DD bands.To increase throughput, the cloning of DD bands is replaced by a gene-specific primer approach, and hybridiza

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Luminal-contact-inhibition of epithelial basal stem cell multipotency in prostate organogenesis and homeostasis

Prostate epithelial basal cells are highly plastic in their luminal differentiation capability.Basal stem cells actively Assessment Tools produce luminal cells during organogenesis, but become restricted in the adult prostate unless receiving oncogenic or inflammatory stimuli.Given that the number of luminal cells increases relative to basal cells

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The Possibility of Pilgrimage in a Scientific World

The paper is a philosophical argument about whether a pilgrimage can be meaningful in a scientific age.Since a scientific world-view rules out many ideas which are traditionally associated with pilgrimage, such as miracles and the effectiveness of prayer, it seems that pilgrimage might be a practice inconsistent with the modern scientific age.Attem

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